Application of Biotechnology in Controlling Aspergillus flavus and Mitigating Aflatoxin Contamination in Peanuts (Arachis hypogaea L.)
Published:
2026-01-19Issue:
Vol. 1 No. 3 (2025): JOURNAL OF MULTIDISCIPLINARY SCIENCE AND NATURAL RESOURCE MANAGEMENTKeywords:
Aflatoksin, Bioteknologi, CRISPR, Gene Silencing, Kacang Tanah, RNAiArticle
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Abstract
Aflatoxin is a carcinogenic mycotoxin produced by the fungi Aspergillus flavus and A. parasiticus. Contamination with this compound is most commonly found in peanuts in tropical regions, including Indonesia. This study aims to evaluate various biotechnological approaches to control A. flavus and reduce aflatoxin contamination in peanuts (Arachis hypogaea L.). The method used was a Systematic Literature Review (SLR) analyzing 11 scientific journals published between 2015 and 2025. The study results showed that Host-Induced Gene Silencing (HIGS) technology can reduce aflatoxin contamination to less than 20 ppb by silencing the aflM and aflP genes. RNA interference (RNAi) technology using the p5XCAPD plasmid successfully inhibited aflatoxin B1 and B2 production by up to 100% in transgenic lines. The CRISPR/Cas9 approach also showed potential for editing aflatoxin biosynthesis genes and increasing plant resistance. Genomic and proteomic approaches have successfully identified key genes such as LOX, NBS-LRR, and PR proteins that play a role in resistance mechanisms. Combining pre- and post-harvest control strategies with biotechnological approaches offers great promise for effectively and sustainably suppressing aflatoxin contamination.
References
Ajmal, M., Bedale, W., Akram, A., & Yu, J.-H. (2022). Comprehensive Review of Aflatoxin Contamination, Impact on Health and Food Security, and Management Strategies in Pakistan. Toxins, 14(12). https://doi.org/10.3390/toxins14120845
Akullo, J. O., Okello, D. K., Mohammed, A., Muyinda, R., Amayo, R., Magumba, D., Gidoi, R., Njoroge, S., & Mweetwa, A. (2025). A Comprehensive Review of Aflatoxin in Groundnut and Maize Products in Africa: Prevalence, Detection and Mitigation Strategies. Journal of Food Quality, 2025(1). https://doi.org/10.1155/jfq/2810946
Arias, R. S., Dang, P. M., & Sobolev, V. S. (2015). RNAi-mediated control of aflatoxins in peanut: Method to analyze mycotoxin production and transgene expression in the peanut/aspergillus pathosystem. Journal of Visualized Experiments, 2015(106). https://doi.org/10.3791/53398
Bhatnagar-Mathur, P., Sunkara, S., Bhatnagar-Panwar, M., Waliyar, F., & Sharma, K. K. (2015). Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops. In Plant Science (Vol. 234, pp. 119–132). Elsevier Ireland Ltd. https://doi.org/10.1016/j.plantsci.2015.02.009
Broto, W. (2018). STATUS CEMARAN DAN UPAYA PENGEDALIAN AFLATOKSIN PADA KOMODITAS SEREALIA DAN ANEKA KACANG TANAH. Jurnal Litbang Pertanian. https://doi.org/10.21082/jp3.v37n2.2018.p81-90
Chowdhury, S., Datta, A., Ferdous, M.-E.-M., & Sinha, D. (2022). Genetic Transformation of Arachis hypogaea Using Novel Genes Conferring Fungal Resistance-A Review. HORIZON E-Plublishing Group. https://plantsciencetoday.online
Gelaye, Y. (2025). Integrated breeding, genomics, and epigenetic approaches enhance aflatoxin resistance in peanut (Arachis hypogaea L.) under pathogen and climate stress. Vegetos. https://doi.org/10.1007/s42535-025-01546-x
Han, S. J., Chae, J., Kim, H. J., Kim, J. H., Chung, Y. S., Karthik, S., & Heo, J. B. (2025). CRISPR-Mediated Genome Editing in Peanuts: Unlocking Trait Improvement for a Sustainable Future. In Plants (Vol. 14, Issue 21). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/plants14213302
Jayaprakash, A., Thanmalagan, R. R., Roy, A., Arunachalam, A., & Lakshmi, P. T. V. (2019). Strategies to understand Aspergillus flavus resistance mechanism in Arachis hypogaea L. In Current Plant Biology (Vol. 20). Elsevier B.V. https://doi.org/10.1016/j.cpb.2019.100123
Khadgi, A., Lekkala, S., Verma, P. K., Puppala, N., & Janga, M. R. (2025). Emerging Strategies for Aflatoxin Resistance in Peanuts via Precision Breeding. In Toxins (Vol. 17, Issue 8). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/toxins17080394
Konatham, A., Gaddam, V. R., & Cochran, D. S. (2026). Progress Roadmap and Information Hub for Purdue University Fort Wayne Student Success. In Lecture Notes in Networks and Systems: Vol. 1605 LNNS. https://doi.org/10.1007/978-3-032-03722-0_10
Pandey, M. K., Kumar, R., Pandey, A. K., Soni, P., Gangurde, S. S., Sudini, H. K., Fountain, J. C., Liao, B., Desmae, H., Okori, P., Chen, X., Jiang, H., Mendu, V., Falalou, H., Njoroge, S., Mwololo, J., Guo, B., Zhuang, W., Wang, X., … Varshney, R. K. (2019). Mitigating aflatoxin contamination in groundnut through a combination of genetic resistance and post-harvest management practices. In Toxins (Vol. 11, Issue 6). MDPI AG. https://doi.org/10.3390/toxins11060315
Prasad, K., Yogendra, K., Sanivarapu, H., Rajasekaran, K., Cary, J. W., Sharma, K. K., & Bhatnagar-Mathur, P. (2023). Multiplexed Host-Induced Gene Silencing of Aspergillus flavus Genes Confers Aflatoxin Resistance in Groundnut. Toxins, 15(5). https://doi.org/10.3390/toxins15050319
Pushkarna, S., Gaba, K., Kharod, S., Kumar, A., Devi, S. U., Suneja, P., & Dang, A. S. (2025). Afatoxigenic Fungi: Human Health Impacts and Control Methods. In Aflatoxigenic Fungi Its Impact on Plant Animal and Human Health. https://doi.org/10.1201/9781003487043-25
Salsabila, A. T., Wardhani, R. M., Chodijayanti, A., Puryani, Damat, & Anggriani, R. (2021). Penetapan Kadar Aflatoksin B1, B2, G1, dan G2 pada Olahan Kacang Tanah dengan Metode HPLC Info Artikel. Jurnal Teknologi Pangan Dan Hasil Pertanian, 16(2), 2021–2022. https://doi.org/http://dx.doi.org/10.26623/jtphp.v16i1
Sharma, K. K., Pothana, A., Prasad, K., Shah, D., Kaur, J., Bhatnagar, D., Chen, Z. Y., Raruang, Y., Cary, J. W., Rajasekaran, K., Sudini, H. K., & Bhatnagar-Mathur, P. (2018). Peanuts that keep aflatoxin at bay: a threshold that matters. Plant Biotechnology Journal, 16(5), 1024–1033. https://doi.org/10.1111/pbi.12846
Wang, X., You, S.-H., Lien, K.-W., & Ling, M.-P. (2019). Using disease-burden method to evaluate the strategies for reduction of aflatoxin exposure in peanuts. Toxicology Letters, 314, 75–81. https://doi.org/10.1016/j.toxlet.2019.07.006
Author Biographies
Auliya Safitri, Program Studi Magister Pertanian Lahan Kering, Pascasarjana Universitas Mataram, Kota Mataram, Nusa Tenggara Barat 83115, Indonesia
Muhammad Sarjan, Program Studi Magister Pertanian Lahan Kering, Pascasarjana Universitas Mataram, Kota Mataram, Nusa Tenggara Barat 83115, Indonesia
Muhammad Taufik Fauzi, Program Studi Magister Pertanian Lahan Kering, Pascasarjana Universitas Mataram, Kota Mataram, Nusa Tenggara Barat 83115, Indonesia
Pending Dadih Permana, Program Studi Magister Pertanian Lahan Kering, Pascasarjana Universitas Mataram, Kota Mataram, Nusa Tenggara Barat 83115, Indonesia
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Copyright (c) 2025 Auliya Safitri, Muhammad Sarjan, Muhammad Taufik Fauzi, Pending Dadih Permana
This work is licensed under a Creative Commons Attribution 4.0 International License.
